Glycine is a competitive antagonist of the TNF receptor mediating the expression of inflammatory cytokines in 3T3-L1 adipocytes

Autor: Santiago Villafaña-Rauda, Fengyang Huang, Karla Aidee Aguayo-Cerón, Rodrigo Romero-Nava, Martha A Ballinas-Verdugo, Gerardo Blancas-Flores, Francisco Javier Alarcón-Aguilar, Abraham Giacoman-Martínez, Fausto Sánchez-Muñoz, Julio César Almanza-Pérez
Rok vydání: 2021
Předmět:
Zdroj: Inflammation Research. 70:605-618
ISSN: 1420-908X
1023-3830
Popis: To determine the involvement of TNF-α and glycine receptors in the inhibition of pro-inflammatory adipokines in 3T3-L1 cells. RT-PCR evidenced glycine receptors in 3T3-L1 adipocytes. 3T3-L1 cells were transfected with siRNA for the glycine (Glrb) and TNF1a (Tnfrsf1a) receptors and confirmed by confocal microscopy. Transfected cells were treated with glycine (10 mM). The expressions of TNF-α and IL-6 mRNA were measured by qRT-PCR, while concentrations were quantified by ELISA. Glycine decreased the expression and concentration of TNF-α and IL-6; this effect did not occur in the absence of TNF-α receptor due to siRNA. In contrast, glycine produced only slight changes in the expression of TNF-α and IL-6 in the absence of the glycine receptor due to siRNA. A docking analysis confirmed the possibility of binding glycine to the TNF-α1a receptor. These findings support the idea that glycine could partially inhibit the binding of TNF-α to its receptor and provide clues about the mechanisms by which glycine inhibits the secretion of pro-inflammatory adipokines in adipocytes through the TNF-α receptor.
Databáze: OpenAIRE
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