Leaf Extract of Osbeckia octandra L. (Heen Bovitiya) Suppresses Human Oral Squamous Cell Carcinoma Cells Migration and Induces Cellular DNA Damage
| Autor: | Sudarma Bogahawaththa, Rasika P. Illeperuma, Suranga P. Kodithuwakku, Madhusha Prasadani |
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| Rok vydání: | 2021 |
| Předmět: |
business.industry
DNA damage Cell migration 030206 dentistry medicine.disease Molecular biology In vitro Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Otorhinolaryngology Apoptosis Cellular dna 030220 oncology & carcinogenesis Carcinoma medicine Surgery Viability assay Oral Surgery Cytotoxicity business |
| Zdroj: | Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. 33:215-220 |
| ISSN: | 2212-5558 |
| DOI: | 10.1016/j.ajoms.2020.09.003 |
| Popis: | Objective The study was designed to investigate the anti-cancer potential of Osbeckia octandra leaf extract using an in vitro cell culture model with YD-38 oral carcinoma cells. Methods Human oral squamous cell carcinoma (OSCC) cells, YD-38 were cultured until sub-confluency and treated with 3 different concentrations (0.3, 3.0 and 30 μg/mL) of freeze-dried water extracts of O. octandra leaves. The effects of the extract on cell viability, migration and DNA damage were assessed. Results The O. octandra concentrations dose and time dependently reduced cell viability. The significantly highest response was observed at 30 μg/mL of concentration (p Conclusion The O. octandra leaf extracts clearly demonstrates an in vitro anti-cancer potential and it can be primarily via the reduction of cell migration and induced cellular DNA damage rather than direct cytotoxicity. Reduction of cell migration potential and DNA damage induction are a vital for inducing the apoptosis of the OSCC. Thus, present data warrant in-depth study to identify the exact bio-active components to establish an effective anti-cancer therapy from O. octandra against oral carcinoma. |
| Databáze: | OpenAIRE |
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