Quantification of Viable but Non-Culturable Cells of Legionella pneumophila
| Autor: | Sébastien P. Faucher, Laam Li, Nilmini Mendis, Rosalie Salus Braun |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
medicine.diagnostic_test biology 030306 microbiology biology.organism_classification Legionella pneumophila Flow cytometry Microbiology Staining 03 medical and health sciences chemistry.chemical_compound chemistry Cell wall integrity medicine Propidium iodide Bacteria 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493990474 |
| Popis: | Legionella pneumophila, among other bacteria, may enter a viable but non-culturable state as a means for survival in stressful conditions. Bacterial cells in the viable but non-culturable state cannot grow on standard medium; however, they continue to exhibit characteristics that are associated with live cells, such as respiration, transcription, and cell wall integrity. The present paper outlines a detailed protocol for the detection of viable but non-culturable L. pneumophila cells via Syto® 9 and propidium iodide staining coupled with flow cytometry. |
| Databáze: | OpenAIRE |
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