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Background: Circular RNAs are a type of closed non-coding RNAs, lack the terminating 5'-capand 3'-polyadenylated tail structures that present in linear RNAs. They have higher biologicalstability because of their resistance to RNA exonucleases and in some tissues; their expressionlevels are ten times higher than other types of RNA. CircRNAs act as biomarkers for variousdiseases including diabetes mellitus as they have been found to affect insulin secretion and β-cellrenewal. Objective: to investigate the role of circRNA (0054633) as a biomarker for pre-diabetesand T2DM. Subjects & Methods: A total number of 149 subjects, selected from endocrinologyunit of Internal Medicine Department, and Munshaat Sultan Family Medicine Clinic Menoufiauniversity hospital, classified into three groups : group I were 55 T2DM patients, group II were 44pre-diabetics and group III including 50 healthy subjects. Expression profile of circRNA(0054633) in plasma of the studied subjects was analyzed by quantitative real- time polymerasechain reaction (Q-PCR). Results: CircRNA (0054633) expression was significantly increasedgradually from controls to the pre-diabetes group to the T2DM group (p |
