| Popis: |
Porcine interferon γ (poIFN-γ) and porcine granulocyte-macrophage colony-stimulating factor(poGM-CSF) are multi-functional cytokines that have previously been shown to exhibit robust antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV). However, it remains to be established as to whether a recombinant poIFN-γ-poGM-CSF fusion protein prepared in a prokaryotic expression system offers value as an adjuvant when administered with the PRRSV killed vaccine (KV). In the present study, a recombinant poIFN-γ-linker-poGM-CSF fusion gene was created via splicing by overlap extension PCR (SOE-PCR) and prepared using an E. coli expression system, after which its adjuvant activity in the context of PRRSV KV administration was assessed. This analysis revealed the successful construction of the poIFN-γ-linker-poGM-CSF fusion gene via SOE-PCR, with recombinant poIFN-γ-linker-poGM-CSF successfully being prepared in E. coli with the pET32a vector. Importantly, the co-administration of poIFN-γ-linker-poGM-CSF and PRRSV KV resulted in a significant increase in neutralizing antibody titers, accelerated viral clearance, reduced clinical symptoms, and enhanced overall protection against HP-PRRSV infection. |
