Curcumin by activation of adenosine A2A receptor stimulates protein kinase a and potentiates inhibitory effect of cangrelor on platelets

Autor:	Stepan Gambaryan, Valentina Shpakova, Alexandra Kharazova, Natalia Rukoyatkina, Igor Mindukshev, Alina Bogoutdinova
Rok vydání:	2022
Předmět:	P2Y receptor Biophysics Adenosine A2A receptor Cell Biology Pharmacology Biochemistry Adenosine chemistry.chemical_compound Cangrelor P2Y12 chemistry Curcumin medicine Platelet activation Protein kinase A Molecular Biology medicine.drug
Zdroj:	Biochemical and Biophysical Research Communications. 586:20-26
ISSN:	0006-291X
DOI:	10.1016/j.bbrc.2021.11.006
Popis:	Curcumin is a natural polyphenol derived from the turmeric plant (Curcuma longa) which exhibits numerous beneficial effects on different cell types. Inhibition of platelet activation by curcumin is well known, however molecular mechanisms of its action on platelets are not fully defined. In this study, we used laser diffraction method for analysis of platelet aggregation and Western blot for analysis of intracellular signaling mechanisms of curcumin effects on platelets. We identified two new molecular mechanisms involved in the inhibitory effects of curcumin on platelet activation. Firstly, curcumin by activation of adenosine A2A receptor stimulated protein kinase A activation and phosphorylation of Vasodilator-stimulated phosphoprotein. Secondly, we demonstrated that curcumin even at low doses, which did not inhibit platelet aggregation, potentiated inhibitory effect of ADP receptor P2Y12 antagonist cangrelor which partly could be explained by activation of adenosine A2A receptor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3998faaf66000607e391d906a714df02 https://doi.org/10.1016/j.bbrc.2021.11.006 Zobrazit plný text záznamu Full Text from ScienceDirect