Quantitative Method of Disulfiram Determination by Thin-Layer Chromatographic Image Analysis Technique

Autor:	Witold Ciesielski, Monika Skowron, Robert Zakrzewski, Żaneta Rembisz
Rok vydání:	2014
Předmět:	Detection limit Chromatography Chemistry Clinical Biochemistry Thin layer chromatographic Disulfiram Analytical chemistry medicine Biochemistry Quantitative analysis (chemistry) Quantitative determination Analytical Chemistry medicine.drug
Zdroj:	Journal of Planar Chromatography – Modern TLC. 27:107-112
ISSN:	1789-0993 0933-4173
DOI:	10.1556/jpc.27.2014.2.7
Popis:	A simple and sensitive thin-layer chromatographic (TLC) method coupled with an image analysis technique was developed for the quantitative determination of disulfiram. Separation was performed on RP-18 plates with methanol—water 90:10 (v/v) as mobile phase. Disulfiram was detected using copper(II) ions (producing a yellow complex). Digital images of TLC plate chromatograms were converted into peak chromatograms using TLSee software, and quantitative analysis was conducted. Linearity was obtained in the range of 1.0–10 µg/spot. The limits of detection and quantification were 0.26 µg/spot and 0.86 µg/spot, respectively. Quantitative determination of disulfiram in Anticol was also performed, with a recovery of 100.3%. The method was applied for the determination of disulfiram in a urine-spiked sample.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::39934d37eba76565fb0809a4e3539cec https://doi.org/10.1556/jpc.27.2014.2.7 Zobrazit plný text záznamu Full text from SpringerLink