Effects of Sinusoidal and Pulsed Magnetic Fields on the Rb-Uptake of Transplanted Chick Embryos

Autor: Jukka Juutilainen, Keijo Saali
Rok vydání: 1988
Předmět:
Zdroj: Journal of Bioelectricity. 7:137-140
ISSN: 0730-823X
Popis: Our previous studies suggested that low-frequency magnetic fields ( LFMFs) can disturb the development of chick embryos (1). Neither the mechanism nor the primary level of the effect is known. The effect seemed independent of the rate of change of the magnetic field, and sinusoidal fields were found to be as effective as pulsed waveforms (2,3). This suggests that the mechanism of the effect is not magnetic induction. The aim of this study was to compare the effect of sinusoidal and pulsed magnetic field (PMF) on the Rb-uptake (Rb as an indicator f or K) of 60-7O-hours-old transplanted chick embryos. The method used was chosen because it is a popular hypothesis that the primary effect of magnetic fields occurs at the cell membrane. The exposure system consisted of a function generator and a power amplifier connected to a mod if fed rectangular Helmholtz coil with width 12.5 cm and length 17 cm. The height of the coil was 2 cm and the distance between the coils in Helmholtz arrangement was 8 cm. Each of the coils had 50 turns of enamelled copper wire (diameter 0.8 mm) and the inductance of the coils connected in parallel was 50.3 pH. In each experiment four similar systems were used, three for exposing and one for shanrexposing the control embryos. The measured background magnetic field strength inside the control system was about 0.5% of that of the exposure systems. The homogeneity of the magnetic field was within 6% inside the exposure volume. The exposure sysras were open so that the air could flow between the coils to establish the same temperature within 0.5'C for the control and exposed embryos.
Databáze: OpenAIRE