Clonal relatedness and plasmid profiling of extensively drug-resistant New Delhi metallo-β-lactamase-producing Klebsiella pneumoniae clinical isolates
| Autor: | Shah Jahan, Muhammad Usman Qamar, Asad Ali Shah, Roua M. Alkufeidy, Hasan Ejaz, Sidrah Saleem, Dunia A. Al Farraj, Noorah A. Alkubaisi, Timothy R. Walsh |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Molecular epidemiology Klebsiella pneumoniae Drug resistance biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses biology.organism_classification Microbiology Minimum inhibitory concentration Colistin medicine Pulsed-field gel electrophoresis Multilocus sequence typing Typing medicine.drug |
| Zdroj: | Future Microbiology. 16:229-239 |
| ISSN: | 1746-0921 1746-0913 |
| DOI: | 10.2217/fmb-2020-0315 |
| Popis: | Aim: Carbapenem-resistant Klebsiella pneumoniae (CR-KP) particularly New Delhi metallo-β-lactamase (NDM) is a serious public health concern globally. The aim of the study to determine the molecular epidemiology of blaNDM-producing clinically isolated K. pneumoniae. Methods: Carbapenem-resistant K. pneumoniae isolates (n = 100) were collected from tertiary care hospital Lahore. Isolates were confirmed by VITEK® 2 system and MALDI-TOF. Minimum inhibitory concentration was performed by VITEK 2 and molecular characterization was done by PCR, PFGE, DNA hybridization and replicon typing. Results: Of 90 MBL-producing K. pneumoniae, 75 were NDM producers; 60 were NDM-1 and 11 NDM-5. A total of 27 K. pneumoniae belonged to ST11 and 14 to ST147. NDM-positive isolates were 100% resistant to β-lactam antibiotics except for colistin. 13.3% isolates carried blaNDM on ∼140 kb plasmids. A total of 32 (52.4%) isolates were positive for IncA/C and 18 (29.5%) IncF/II. Conclusion: The extensively resistant lineage of NDM-producing K. pneumoniae is prevalent in the clinical setting. |
| Databáze: | OpenAIRE |
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