In vitroregeneration and transformation in chilli pepper (Capsicum annuum L.)

Autor:	Gowda Ramanjini, G. V. S. Saiprasad, Anand Lalitha, T. K. S. Gowda, S.T. Shivegowda, J.B. Mythili
Rok vydání:	2002
Předmět:	Reporter gene food and beverages Kanamycin Agrobacterium tumefaciens Horticulture Biology biology.organism_classification Transformation (genetics) chemistry.chemical_compound Murashige and Skoog medium chemistry Botany Pepper Genetics medicine Zeatin medicine.drug Explant culture
Zdroj:	The Journal of Horticultural Science and Biotechnology. 77:629-634
ISSN:	2380-4084 1462-0316
Popis:	SummaryRegeneration of well-developed shootlets from cotyledonary explants of chilli `Pusa Jwala' and `G4' was obtained in MS medium supplemented with (9–18.µM) zeatin in combination with GA3 (2.89.µM). The elongated shoots could be rooted successfully on IBA containing MS medium. Cotyledonary explants of `Pusa Jwala' were transformed with Agrobacterium tumefaciens st C58 containing binary vector pGV1040 harbouring the two reporter genes npt II and GUS. The transformed shoots were selected on regeneration medium containing kanamycin (100 mg l–1) and cefotaxime (500 mg l–1) and subsequently rooted in the presence of kanamycin (75 mg l–1). The presence of the transgene was confirmed through histochemical staining of GUS, polymerase chain reaction (PCR) and Southern hybridization analysis of npt II gene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3987ee583c9510603e87dad2ec66aabe https://doi.org/10.1080/14620316.2002.11511549 Zobrazit plný text záznamu