| Autor: |
Albano H. Tenaglia, Lucas A. Luján, Diego N. Ríos, Victor Midlej, Paula A. Iribarren, Cecilia R. Molina, M. Agustina Berazategui, Alessandro Torri, Alicia Saura, Damián O. Peralta, Macarena Rodríguez-Walker, Elmer A. Fernández, Juan P. Petiti, Marianela C. Serradell, Pablo R. Gargantini, Vanina E. Alvarez, Wanderley de Souza, Hugo D. Luján |
| Rok vydání: |
2022 |
| Popis: |
The genomes of most protozoa encode families of variant surface antigens, whose mutually exclusive changes in expression allow parasitic microorganisms to evade the host immune response1,2. It is widely assumed that antigenic variation in protozoan parasites is accomplished by the spontaneous appearance within the population of cells expressing antigenic variants that escape antibody-mediated cytotoxicity1,2. Here we show, bothin vitroand in animal infections, that antibodies to Variant-specific Surface Proteins (VSPs) of the intestinal parasiteGiardia lambliaare not cytotoxic, inducing instead VSP clustering into liquid-ordered phase membrane microdomains that trigger a massive release of microvesicles carrying the original VSP and switch in expression to different VSPs by a calcium-dependent mechanism. Surface microvesiculization and antigenic switching are also stimulated whenTrypanosoma bruceiandTetrahymena thermophilaare confronted to antibodies directed to their GPI-anchored variable surface glycoproteins. This novel mechanism of surface antigen clearance throughout its release into microvesicles coupled to the stochastic induction of new phenotypic variants not only changes the current paradigm of spontaneous antigenic switching but also provides a new framework for understanding the course of protozoan infections as a host/parasite adaptive process. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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