A study on the combined effects of carbon and nitrogen source on high added value products synthesis by Nannochloropsis oceanica CCMP1779 using response surface methodology
| Autor: | Theodore G. Sotiroudis, Kalliopi P. Balta-Brouma, Theopisti Lymperopoulou, Dimitris Kekos, Diomi Mamma, Maria G. Savvidou, Fragiskos N. Kolisis |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Central composite design chemistry.chemical_element Biomass Bioengineering 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology chemistry.chemical_compound 010608 biotechnology Organic chemistry Response surface methodology Food science 0105 earth and related environmental sciences chemistry.chemical_classification food and beverages Eicosapentaenoic acid Nitrogen Oleic acid chemistry lipids (amino acids peptides and proteins) Agronomy and Crop Science Carbon Food Science Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 10:298-307 |
| ISSN: | 1878-8181 |
| DOI: | 10.1016/j.bcab.2017.04.006 |
| Popis: | Response surface methodology (RSM) was used to evaluate the combined effect of the initial carbon (NaHCO 3 ) and nitrogen (NaNO 3 ) source concentration on the synthesis of high added value products by Nannochloropsis oceanica CCMP1779, applying a 2 2 full-factorial central composite design with four axial points. Biomass and lipid production were influenced by the variations of the tested parameters. Highest biomass production (0.581 g/L) and specific growth rate (0.279 d −1 ) were obtained at high carbon concentration, while low nitrogen concentration favors the lipids accumulation (highest lipid content 44.65 g/100 g DCW). The fatty acids (FAs) profile of N. oceanica CCMP1779 was dominated by saturated (SFAs) and monounsaturated fatty acids (MUFAs), comprising 88–95% of the total FAs, while polyunsaturated acids (PUFAs) accounted for 5–11%. Palmitic (C16:0), palmitoleic (C16:1 n-7), oleic acid (C18:1 n-9), myristic (C14:0) and eicosapentaenoic acid (C20:5 n-3) (EPA) were the major FAs detected and their content depended on the (NaHCO 3 ) and nitrogen (NaNO 3 ) combination. Optimization of through RSM indicated that a combination of 1.3 g/L NaHCO 3 and 0.06 g/L NaNO 3 maximizes the FAs accumulation and MUFAs content, while the above conditions have the exact opposite effect on PUFAs and EPA content. |
| Databáze: | OpenAIRE |
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