Pyrazinamide susceptibility testing in Mycobacterium tuberculosis using the fast resazurin microtiter assay plate
| Autor: | Regiane Bl Scodro, L D Ghiraldi-Lopez, Vera Ld Siqueira, R Z Pina, Fernando Rogério Pavan, R. F. Cardoso, Paula Aline Zanetti Campanerut-Sá, Katiany Rizzieri Caleffi-Ferracioli |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Susceptibility testing Tuberculosis biology business.industry 030106 microbiology Resazurin Drug resistance Pyrazinamide biology.organism_classification medicine.disease Microbiology Mycobacterium tuberculosis 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration Infectious Diseases chemistry Mic values Medicine business medicine.drug |
| Zdroj: | The International Journal of Tuberculosis and Lung Disease. 20:1535-1538 |
| ISSN: | 1815-7920 1027-3719 |
| DOI: | 10.5588/ijtld.16.0304 |
| Popis: | Setting Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, PR, Brazil. Objective To evaluate the performance of the resazurin microtiter assay (REMA) plate at pH 5.5 in detecting Mycobacterium tuberculosis susceptibility to pyrazinamide (PZA). Design The minimal inhibitory concentration (MIC) of PZA in M. tuberculosis H37Rv and M. bovis AN5 reference strains and in 34 clinical M. tuberculosis isolates (26 PZA-susceptible and eight PZA-resistant) was determined using REMA at pH 5.5 and compared to REMA at pH 6.0. Results REMA at pH 5.5 was helpful in discriminating PZA-susceptible from resistant M. tuberculosis isolates when ⩿50 μg/ml PZA was considered as the cut-off for PZA susceptibility. Furthermore, it provided results in 8 days. However, two PZA-resistant isolates failed to grow at pH 5.5. Conclusion As the REMA method is rapid, inexpensive, easy to perform and read, it would be of great usefulness in low-income countries for detecting PZA-resistant M. tuberculosis. REMA at pH 5.6-5.9 should be evaluated on an extended panel of clinical M. tuberculosis isolates with a greater range of MIC values in different laboratories for a better understanding of its utility in differentiating PZA-resistant from PZA-susceptible isolates. |
| Databáze: | OpenAIRE |
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