Detection of antibody-antigen reaction by silicon nitride slot-ring biosensors using protein G
| Autor: | Yoshiteru Amemiya, Anna Hirowatari, Tomoya Taniguchi, Takeshi Ikeda, Shin Yokoyama, Masataka Fukuyama, Akio Kuroda |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Ring (chemistry) 01 natural sciences 010309 optics Slot-waveguide Resonator chemistry.chemical_compound Optics 0103 physical sciences Molecule Electrical and Electronic Engineering Physical and Theoretical Chemistry 010302 applied physics biology business.industry technology industry and agriculture Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Silicon nitride chemistry biology.protein Optoelectronics Protein G business Biosensor Temperature coefficient |
| Zdroj: | Optics Communications. 365:16-23 |
| ISSN: | 0030-4018 |
| DOI: | 10.1016/j.optcom.2015.11.068 |
| Popis: | Biosensors using ring resonators with silicon nitride (SiN) slot waveguides have been fabricated. The temperature coefficient of the resonance wavelength of the SiN resonator is 0.006 nm/°C, which is one order of magnitude smaller than that of Si. The sensitivity of the biosensor has been improved by using slot waveguide together with Si-binding protein (designated as Si-tag), which bonds to SiN or SiO 2 surface, as an anchoring molecule to immobilize bioreceptors on the SiN rings in an oriented manner. Furthermore, the protein G, which strongly bonds to many kinds of mammalian antibodies only by mixing the antibody solution, is used to efficiently immobilize the antigen on the sensor surface. By means of these devises the sensitivity of the biosensor has been improved by factor of 10–100 compared with that of normal Si ring resonator sensors without slot. Then the detection of prostate specific antigen (PSA) with the sensitivity of ~1×10 −8 g/ml, which is the concentration of strongly suspicious for the prostate cancer, has been achieved. |
| Databáze: | OpenAIRE |
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