Expression and Purification of Recombinant CDKs: CDK7, CDK8, and CDK9
| Autor: | Reena Grittle Pinhero, Krassimir Yankulov |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Regulation of gene expression biology Kinase Cellular differentiation Cell cycle Cell biology 03 medical and health sciences 030104 developmental biology Biochemistry Cyclin-dependent kinase biology.protein Cyclin-dependent kinase 8 Cyclin-dependent kinase 9 Cyclin-dependent kinase 7 |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493929252 |
| DOI: | 10.1007/978-1-4939-2926-9_3 |
| Popis: | Cyclin-dependent kinases have established roles in the regulation of cell cycle, in gene expression and in cell differentiation. Many of these kinases have been considered as drug targets and numerous efforts have been made to develop specific and potent inhibitors against them. The first step in all of these attempts and in many other biochemical analyses is the production of highly purified and reliable kinase, most frequently in a recombinant form. In this chapter we describe our experience in the cloning, expression, and purification of CDKs using CDK7/CycH, CDK8/CycC, and CDK9/CycT1 as an example. |
| Databáze: | OpenAIRE |
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