| Autor: |
Chang Yeok Moon, Byeong Hee Kang, Woon Ji Kim, Sreeparna Chowdhury, Sehee Kang, Jeong-Dong Lee, Soon-Jae Kwon, Han-Yong Kim, Hyeon-Seok Lee, Bo-Keun Ha |
| Rok vydání: |
2023 |
| DOI: |
10.21203/rs.3.rs-2730946/v1 |
| Popis: |
Soil salinity is a worldwide problem that reduces crop yields, but the development of salt-tolerant crops can help overcome this challenge. This study was conducted with the purpose of evaluating the physiological and genetic characteristics of a new salt-tolerant mutant KA-1285 developed using gamma-ray irradiation in soybean (Glycine max L.). The physiological responses of KA-1285 were compared with salt-sensitive and salt-tolerant genotypes after treatment with 150 mM NaCl for two weeks. In addition, a major salt tolerance quantitative trait locus (QTL) was identified on chromosome 3 in a study using the Daepung X KA-1285 169 F2:3 population, and a specific deletion was identified in Glyma03g171600 (Wm82.a2.v1.1) near the QTL region based on re-sequencing analysis. A kompetitive allele-specific PCR (KASP) marker was developed based on the deletion of Glyma03g171600 that distinguished the wild-type and mutant alleles. Through the analysis of gene expression patterns, it was confirmed that Glyma03g171700 (Wm82.a2.v1.1) is a major gene that controls salt tolerance functions in Glyma03g32900 (Wm82.a1.v1.1). These results suggest that the gamma-ray-induced mutant KA-1285 has the potential to be employed for the development of a salt-tolerant cultivar and provide useful information for genetic research related to salt tolerance in soybeans. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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