Molecular Epidemiology and Characterization of Genotypes of Acinetobacter baumannii Isolates from Regions of South China
Autor: | Ruowang Pan, Huiguang Yi, Liqiang Chen, Li Zong, Jianchao Ying, Jinsong Li, Cong Cheng, Qiyu Bao, Junwan Lu, Liyan Ni, Zuyuan Xu, Teng Xu, Peizhen Li, Kunpeng Li, Jun Ying, Huifen Tou, Chuanxin Zhu, Ailing Li |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Microbiology (medical) biology Molecular epidemiology medicine.drug_class 030106 microbiology Antibiotics General Medicine Drug resistance biology.organism_classification Subtyping Acinetobacter baumannii Microbiology 03 medical and health sciences Infectious Diseases Genotype Pulsed-field gel electrophoresis medicine Multilocus sequence typing |
Zdroj: | Japanese Journal of Infectious Diseases. 69:180-185 |
ISSN: | 1884-2836 1344-6304 |
Popis: | The aim of this study was to analyze the molecular epidemiologic characteristics of Acinetobacter baumannii. A total of 398 isolates were collected in 7 regions of South China from January to June of 2012. Drug sensitivity was tested toward 15 commonly used antibiotics; thus, 146 multi-drug-resistant strains (resistant to more than 7 drugs) were identified, representing 36.7% of all isolates. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular subtyping. According to the PFGE results (with a cutoff of 70% similarity for the DNA electrophoretic bands), 146 strains were subdivided into 15 clusters, with cluster A being the largest (33.6%, distributed in all districts except Jiaxing). Cluster B was also widespread and included 14.4% of all strains. In addition, MLST results revealed 11 sequence types (ST), with ST208 being the most prevalent, followed by ST191 and ST729. Furthermore, 4 novel alleles and 6 novel STs were identified. Our results showed that multi-drug-resistant A. baumannii in South China shares the origin with other widespread strains in other countries. The nosocomial infections caused by A. baumannii have been severe in South China. Continuous monitoring and judicious antibiotic use are required. |
Databáze: | OpenAIRE |
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