Separation and characterization of the tryptic peptide mapping of recombinant bovine growth hormone by reversed-phase high-performance liquid chromatography electrospray mass spectrometry
| Autor: | Douglas E. Kiehl, Jen P. Chang, Allison S. Kennington |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
chemistry.chemical_classification
Chromatography Protein mass spectrometry Molecular mass Chemistry Electrospray ionization Organic Chemistry Peptide High-performance liquid chromatography Sample preparation in mass spectrometry Analytical Chemistry law.invention law Recombinant DNA Bovine somatotropin Spectroscopy |
| Zdroj: | Rapid Communications in Mass Spectrometry. 11:1266-1270 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/(sici)1097-0231(199708)11:12<1266::aid-rcm977>3.0.co;2-3 |
| Popis: | Reversed phase high-performance liquid chromatography (RPHPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) was used to separate and characterize the peptides resulting from the tryptic cleavage of somidobove, a recombinant bovine growth hormone. The tryptic digestion of somidobove was carried out at room temperature for 15 hours. The tryptic peptides were separated on a Zorbax SB300 C8 column with trifluoroacetic acid--acetonitrile gradient elution and characterized by LC/ESI-MS. Resulting single and combined peptide fragments were characterized by comparing the observed molecular mass with the calculated mass for these fragments. |
| Databáze: | OpenAIRE |
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