Improved Detection of Little Cherry Virus-2 Using a Hydrolysis Probe to Manage the Pacific Northwest Little Cherry Disease Epidemic

Autor:	Madalyn K. Shires, Alice A. Wright, Scott J. Harper
Rok vydání:	2022
Předmět:	Plant Science Agronomy and Crop Science
Zdroj:	Plant Disease. 106:1875-1881
ISSN:	1943-7692 0191-2917
DOI:	10.1094/pdis-08-21-1769-re
Popis:	Little cherry virus-2 (LChV-2) is a viral pathogen that is reaching epidemic levels in Washington State. This virus is insect vectored and has significant impacts on sweet cherry production. To aid growers in making informed management decisions, we sought to develop a diagnostic assay to better detect isolates of LChV-2 currently found in Washington, allowing more accurate estimations of disease occurrence. This study showed that there were two distinct genotypes of LChV-2 present in Washington State. This information was used to develop an up-to-date reverse transcription real-time quantitative PCR assay, which was then optimized, validated, and compared with four previously published assays of a panel of field samples. This comparison demonstrated that the newly developed assay provided greater sensitivity, accurately detecting
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::390e07404f1a5b5b02df23f9b6e1bcab https://doi.org/10.1094/pdis-08-21-1769-re Zobrazit plný text záznamu