A novel true volumetric method for the determination of residual leucocytes in blood components
| Autor: | K. Tapernon, Uwe Cassens, Burkhard Greve, Walter Sibrowski, B. Nave, Wolfgang Göhde, Erhard Severin |
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| Rok vydání: | 2002 |
| Předmět: |
Detection limit
medicine.medical_specialty Chromatography business.industry Fluorescence spectrometry Automatic processing Hematology General Medicine Residual Surgery Red blood cell medicine.anatomical_structure hemic and lymphatic diseases Blood Component Transfusion Cost control Medicine Platelet concentrate business circulatory and respiratory physiology |
| Zdroj: | Vox Sanguinis. 82:198-206 |
| ISSN: | 0042-9007 |
| DOI: | 10.1046/j.1423-0410.2002.00176.x |
| Popis: | BACKGROUND AND OBJECTIVES Accurate determination of residual leucocytes [white blood cells (WBC)] in blood components is of high clinical importance. To date, several labour-intensive, time-consuming or expensive techniques have been used for this purpose. MATERIALS AND METHODS A method for the determination of residual WBC is described using a novel low-cost flow-cytometric cell counter and analyser (CCA). The DNA in WBC was stained using 4'-6-diamidino-2-phenylindole (DAPI) and WBC were automatically analysed by true volumetric counting of 200-microl samples (prepared from a 20-microl undiluted sample). RESULTS Dilution experiments over a range of 0.5-50 WBC/microl showed a linearity of r = 0.998. The detection limit of this method was 0.83 WBC/microl of red blood cell concentrate (RCC) and 0.67 WBC/microl of platelet concentrate (PC), with an accuracy of 95.5%. CONCLUSION Residual WBC (< 1 WBC/microl) can be accurately counted using the CCA within 2 min and at a total cost of less than euro 1 per sample. |
| Databáze: | OpenAIRE |
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