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Summary Root calli of three legumes — Arachis hypogaea, Cajanus cajan and Cicer arietinum were inoculated with homologous rhizobia. Measurable nitrogenase activity was detected when inoculated calli Arachis , Cajanus and Cicer were tested. Light microscopic observations of transections oe inoculated calli revealed bacterial penetration in the form of infection thread-like cells. In Cicer , characteristics noduar outgrowths were seen whose cells where also filled with bacteria. None of these observations were made in uninoculated calli indicating the features of possible symbiosis in legume root calli upon inoculation with Rhizobium . |
