In Situ Localization of Small RNAs in Plants
| Autor: | Marja C.P. Timmermans, Cristina F. Marco, Damianos S. Skopelitis |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
In situ 0303 health sciences Small RNA Oligonucleotide food and beverages Computational biology In situ hybridization Biology Plant biology 01 natural sciences Plant tissue 03 medical and health sciences microRNA Locked nucleic acid 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493990412 |
| Popis: | Small RNAs have vital roles in numerous aspects of plant biology. Deciphering their precise contributions requires knowledge of a small RNA's spatiotemporal pattern of accumulation. The in situ hybridization protocol described here takes advantage of locked nucleic acid (LNA) oligonucleotide probes to visualize small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within 2-6 days. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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