Neurogenic airway inflammation induced by repeated intra-esophageal instillation of HCl in guinea pigs
| Autor: | Nanshan Zhong, Wei Luo, Chunli Liu, Ru-chong Chen, Kefang Lai |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Neurogenic inflammation Lung business.industry Immunology Substance P Nodose Ganglion respiratory system respiratory tract diseases Vagus nerve Guinea pig chemistry.chemical_compound medicine.anatomical_structure chemistry Anesthesia medicine Immunology and Allergy Neurokinin A Neurokinin B business |
| Zdroj: | Inflammation. 36:493-500 |
| ISSN: | 1573-2576 0360-3997 |
| DOI: | 10.1007/s10753-012-9570-2 |
| Popis: | This study was conducted to investigate if repeated intra-esophageal acid administrations may induce neurogenic inflammation in the airways and nodose ganglion in a guinea pig model. Guinea pigs were sedated and perfused with 0.1 N HCl in the distal esophagus via a nasoesophageal catheter for 14 consecutive days. Substance P (SP), neurokinin A (NKA), neurokinin B (NKB), and calcitonin gene-related peptide concentration were measured by ELISA or radioimmunoassay. Neuropeptide expression in the airways and nodose ganglion was detected by immunohistochemistry and assessed semi-quantitatively. Inflammation was found in the trachea and bronchi. There was a threefold increase in substance P concentration in the trachea, main bronchi, and lung homogenate and a twofold increase in NKA and NKB concentration in the main bronchi, lung homogenate, and bronchial alveolus lavage fluid, respectively. The SP and NKA expressions in the airways and nodose ganglion were also significantly increased. Chronic intra-esophageal acid instillation induces significant neurogenic inflammation in the airways and nodose ganglion in the vagus nerve in guinea pigs. |
| Databáze: | OpenAIRE |
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