Preliminary activation of lymphocytes with mitogen as the condition for manifestation of ability of Fab-fragments of normal IgG to inhibit lymphocyte transformation
| Autor: | G. U. Margulis, F. S. Baranova, A. Ya. Kul'berg |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Blast transformation
Lymphocyte Stimulation General Medicine Biology Inhibitory postsynaptic potential Molecular biology General Biochemistry Genetics and Molecular Biology On cells medicine.anatomical_structure Biochemistry Lymphocyte transformation Mitogen-activated protein kinase Fab Fragments medicine biology.protein |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 87:168-170 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00833980 |
| Popis: | Inhibition of blast-transformation of human lymphocytes, induced by phytohemagglutinin (PHA), with the aid of F(ab′)2-fragment of rabbit IgG was studied. Preincubation of lymphocytes with F(ab′)2-fragments for 24 h at 37 and 4°C followed by change of medium and the addition of PHA did not affect the intensity of blast transformation. However, the F(ab′)2-fragment could depress blast-transformation of lymphocytes if removed from the medium 24 and 48 h after the beginning of stimulation. It can be concluded from these results that the F(ab′)2-fragment interacts only with cells activated by the phytomitogen and exhibits its inhibitory action on cells already bound with PHA. |
| Databáze: | OpenAIRE |
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