Autor:	M. I. Youshko, Vytas K. Švedas, A. L. Bukhanov
Rok vydání:	2003
Předmět:	Chemistry Stereochemistry Kinetics Kinetic scheme General Medicine Kinetic energy Biochemistry Penicillin Active center Nucleophile Computational chemistry medicine Bioorganic chemistry Enzyme kinetics medicine.drug
Zdroj:	Biochemistry (Moscow). 68:334-338
ISSN:	0006-2979
DOI:	10.1023/a:1023014519139
Popis:	The influence of the external nucleophile (6-aminopenicillanic acid) on the kinetics of the penicillin acylase-catalyzed acyl transfer reactions was studied using a highly sensitive spectrophotometric assay. An adequate kinetic scheme is suggested based on kinetic analysis of the experimental dependencies of the kcat and Km values on the nucleophile concentration. The proposed kinetic scheme has been verified by a quantitative description of the above-mentioned experimental dependencies using the set of kinetic parameters obtained from independent experiments. Such an approach can be used for modeling of different penicillin acylase-catalyzed acyl transfer reactions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::38d0c35b201fc707d4333596e984e2dc https://doi.org/10.1023/a:1023014519139 Zobrazit plný text záznamu Full text from SpringerLink