Electrochemical detection of DNA methylation using a glassy carbon electrode modified with a composite made from carbon nanotubes and β-cyclodextrin
| Autor: | Fang Wang, Lili Wang, Zilin Chen, Feiyue Yu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Pyrimidine
Guanine Inorganic chemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Thymine Nucleobase chemistry.chemical_compound 5-Methylcytosine chemistry Saturated calomel electrode Electrochemistry Organic chemistry General Materials Science Electrical and Electronic Engineering 0210 nano-technology DNA Cytosine |
| Zdroj: | Journal of Solid State Electrochemistry. 20:1263-1270 |
| ISSN: | 1433-0768 1432-8488 |
| DOI: | 10.1007/s10008-016-3122-x |
| Popis: | We describe a method for detecting DNA methylation. It is based on direct oxidation of DNA bases at a glassy carbon electrode (GCE) modified with film of a multiwalled carbon nanotube-β-cyclodextrin composite. This nano-structured film causes a strong enhancement on the oxidation current of DNA bases due to its large effective surface area and extraordinary electronic properties. Well-defined peaks were obtained as a result of electro-oxidation of guanine (at 0.67 V), adenine (at 0.92 V), thymine (at 1.11 V), cytosine (at 1.26 V), and 5-methylcytosine (at 1.13 V; all data vs. saturated calomel electrode (SCE)). The potential difference between 5-methylcytosine and cytosine (130 mV) is large enough to enable reliable simultaneous determination and analysis. The interference by thymine can be eliminated by following the principle of complementary pairing between purine and pyrimidine bases in DNA. The modified electrode was successfully applied to the evaluation of 5-methylcytosine in a fish sperm DNA, the methylation level of cytosine was found to be 7.47 %, and the analysis process took less than 1 h. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink