| Autor: |
Lokanath Satish, Mihir Rabha, N. Chandrakanth, Pooja Makwana, V. Sivaprasad |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Methods in Microbiology ISBN: 9780128211458 |
| DOI: |
10.1016/bs.mim.2021.04.004 |
| Popis: |
Nosema bombycis is an obligate intracellular parasite infecting Bombyx mori to cause pebrine disease. The disease is transmitted transovarially and spreads faster in silkworm seed multiplication farms. Pebrine is the only quarantine requirement in silkworm egg production and is usually monitored by mother moth examination and light microscopy for characteristic spores. Practically spore detection is little technical, laborious and the life cycle stages of N. bombycis could also be visualized by microscopy. However, routine monitoring of pebrine disease through light microscopy is limited by specificity and sensitivity. The more practical and feasible diagnostic methods for pebrine monitoring have been met with the development of immunodiagnostic and nucleic acid based detection techniques. Immunodiagnostic assays such as latex agglutination and ELISA employing polyclonal and monoclonal antibodies were developed for the diagnosis and characterization of microsporidian infections. However, false positives and lower level infections made immunoassays to be less-reliable. Nucleic acid-based detection methods utilizing DNA sequences specific to N. bombycis could detect lower levels of infection specifically. Major DNA diagnostics to detect N. bombycis include PCR, Loop-Mediated Isothermal Amplification (LAMP) and lateral flow assays which are high-throughput and quantitative, also enabling simultaneous detection for multiple diseases. Molecular diagnostic techniques are more advantageous than microscopy due to increased sensitivity, specificity, faster and easy result interpretations. The molecular diagnostic tests described for the identification of microsporidia infecting mulberry silkworm are provided in this chapter. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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