Chromatographic Behavior of Proteins on Stationary Phase with Aminocarboxy Ligand
| Autor: | Qing-Yuan Sun, Ju Mingyang, Xiao-Gang Wang, Guo-Liang Chen, Mei Shi, Bin Chen, Jian-Bin Zheng, Rong Li |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Chromatography Silica gel Ligand Inorganic chemistry Salt (chemistry) chemistry.chemical_element General Chemistry respiratory system Copper Matrix (chemical analysis) Metal chemistry.chemical_compound chemistry Stationary phase visual_art visual_art.visual_art_medium Chelation |
| Zdroj: | Bulletin of the Korean Chemical Society. 32:590-594 |
| ISSN: | 0253-2964 |
| DOI: | 10.5012/bkcs.2011.32.2.590 |
| Popis: | An aminocarboxy aspartic acid-bonded silica (Asp-Silica) stationary phase was synthesized using L-aspartic acid as ligand and silica gel as matrix. The standard protein mixtures were separated with prepared chromatographic column. The effects of solution pH, salt concentration and metal ion on the retention of proteins were examined, and also compared with traditional iminodiacetic acid-bonded silica (IDA-Silica) column. The results show that Asp-Silica column exhibited an excellent separation performance for proteins. The retention of proteins on Asp-Silica stationary phase was consistent with electrostatic characteristic of cation-exchange. The stationary phase displayed typical metal chelate property after fixing copper ion (II) on Asp-Silica. Under competitive eluting condition, protein mixtures were effectively isolated. Asp ligand showed better ion-exchange and metal chelating properties as compared with IDA ligand. |
| Databáze: | OpenAIRE |
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