miR-30a-3p Targets MAD2L1 and Regulates Proliferation of Gastric Cancer Cells

Autor: Wanjuan Xue, Juan Du, Jing Yan, Dan Li, Jing Zhang, Jing He, Yu Wang, Jiming Han, Yi Gao, Haiyan Shi, Fenghui Wang, Yun Feng, Yameng Wei, Yani Chen, Huahua Zhang
Rok vydání: 2019
Předmět:
Zdroj: OncoTargets and Therapy. 12:11313-11324
ISSN: 1178-6930
DOI: 10.2147/ott.s222854
Popis: Purpose This study was done to investigate the inhibition effects of miR-30a-3p on mitotic arrest deficient 2 like 1 (MAD2L1) expression and the proliferation of gastric cancer cells. Patients and methods Cluster analysis and the TCGA database were used to screen the key genes highly expressed in gastric cancer. Based on the LinkedOmics website, the correlation between the miR-30a-3p and the cell cycle-related target gene MAD2L1 in gastric cancer was analyzed. The mRNA and protein expression levels were detected with the quantitative real-time PCR and Western blot analysis. The cell proliferation and cell cycle were also detected and analyzed. Results Bioinformatics analysis showed that MAD2L1 was highly expressed in tumor tissues compared with normal tissues. Compared with normal tissues, the miR-30a-3p was significantly decreased in the gastric cancer tissues. Moreover, MAD2L1 was significantly negatively correlated with the miR-30a-3p expression. Furthermore, over-expression of miR-30a-3p decreased the expression of MAD2L1 at the protein level, which inhibited the proliferation of AGS and BGC-823 gastric cancer cells. In addition, the cell cycles of AGS and BGC-823 cells were arrested at the G0/G1 phase. Conclusion MAD2L1 is a pro-oncogene which is up-regulated in gastric cancer. The miR-30a-3p can down-regulate the MAD2L1 expression, inhibiting the proliferation of gastric cancer cells and affect the cell cycle.
Databáze: OpenAIRE
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