Preparation and comparative characterization of keratin–chitosan and keratin–gelatin composite scaffolds for tissue engineering applications
| Autor: | P. Neela Kanta Reddy, Ramadhar Kumar, P.K. Sehgal, Prachi Kakkar, D. Vijaya Ramesh, R. Sripriya, S. Balaji |
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| Rok vydání: | 2012 |
| Předmět: |
Scaffold
Materials science food.ingredient Biocompatibility technology industry and agriculture Bioengineering Gelatin Artificial skin Biomaterials Chitosan chemistry.chemical_compound food Tissue engineering chemistry Chemical engineering Mechanics of Materials Ultimate tensile strength medicine Swelling medicine.symptom Composite material |
| Zdroj: | Materials Science and Engineering: C. 32:975-982 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2012.02.023 |
| Popis: | We report fabrication of three dimensional scaffolds with well interconnected matrix of high porosity using keratin, chitosan and gelatin for tissue engineering and other biomedical applications. Scaffolds were fabricated using porous Keratin–Gelatin (KG), Keratin–Chitosan (KC) composites. The morphology of both KG and KC was investigated using SEM. The scaffolds showed high porosity with interconnected pores in the range of 20–100 μm. They were further tested by FTIR, DSC, CD, tensile strength measurement, water uptake and swelling behavior. In vitro cell adhesion and cell proliferation tests were carried out to study the biocompatibility behavior and their application as an artificial skin substitute. Both KG and KC composite scaffolds showed similar properties and patterns for cell proliferation. Due to rapid degradation of gelatin in KG, we found that it has limited application as compared to KC scaffold. We conclude that KC scaffold owing to its slow degradation and antibacterial properties would be a better substrate for tissue engineering and other biomedical application. |
| Databáze: | OpenAIRE |
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