Copolymers and enzymes blended as LB films changing the bioelectronics properties of interfaces
| Autor: | Mauro Aquiles La-Scalea, Luciano Caseli, Camila Gouveia Barbosa, Laura Oliveira Péres |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Bioelectronics Materials science Supramolecular chemistry chemistry.chemical_element 02 engineering and technology Polymer 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Colloid and Surface Chemistry chemistry Chemical engineering Monolayer Materials Chemistry Copolymer Physical and Theoretical Chemistry 0210 nano-technology Platinum Biosensor Biotechnology |
| Zdroj: | Colloid and Interface Science Communications. 27:40-44 |
| ISSN: | 2215-0382 |
| Popis: | This paper describes Langmuir monolayers of the polymer poly[(9,9-dioctylfluorene)-co-thiophene] (PDOF-co-Th) serving as matrix for urease incorporation and transferred to platinum supports as Langmuir-Blodgett films. The supramolecular structure forming highly organized films not only tuned the catalytic activity of urease, but also helped to prove the viability of the system to sensing urea as demonstrated with current-voltage and constant voltage measurements, exhibiting regular and distinctive output signals over all concentrations (from 1 × 10−3 to 1.5 × 10−2 mol/L) as demonstrated in this work. These results are related to the synergism between the compounds on the active layer, leading to a surface molecular arrangement which allowed fast analyte diffusion owing to an adequate molecular accommodation, taking advantage of the urease activity and of the properties of the polymer as molecular accommodator. This work then demonstrates the feasibility of employing LB films composed of copolymers and urease as electrochemical devices for biosensing applications as a proof-of-concept experiment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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