| Autor: |
Mateo Pineda Quintero, Vivian Andrea Talero Nino, Juan C. Briceño, Laura Lorena Cardenas Bonilla, Juliana Jaramillo Escobar, Carolina Muñoz Camargo, Julieth Paola Oliveros Diaz |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
2018 IX International Seminar of Biomedical Engineering (SIB). |
| Popis: |
In the U.S. more than 6 million patients suffer from chronic wounds (e.g., diabetic, pressure or vascular ulcers), which represent a major public health issue and an important healthcare expenditure. Chronic deep wounds lose the ability to repair without the external signals responsible for enhancing extracellular matrix (ECM) restoration and promoting sustained healing. We have previously prepared Small Intestinal Submucosa-Chitosan (SIS-Ch) and Small Intestinal SubmucosaHyaluronic Acid (SIS-HA) 3D scaffolds, in the form of porous sponges and hydrogels. Physicochemical and mechanical properties in vitro confirmed their potential as wound dressings. Here we conducted in vitro biological tests of SIS-Ch (n=3) and SIS-HA (n=3) scaffolds that included MTT assays to determined cytotoxicity and hemolysis assay to assess the tendency to damage red blood cells. Cell viability of SIS-Ch and SIS-HA sponges exceeded 80%. Nevertheless, SIS-HA hydrogels exhibited the greatest cell viability values of all formulations most likely due to the HA natural function in extracellular matrix that promotes cell migration and proliferation. The presence of Ch and HA in the scaffolds correlated with higher cell metabolic rates when compared with formulations based on SIS only (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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