O049 Interleukin-16 in flush effluent fluid as a biomarker for kidney viability prior to transplantation

Autor: J Dobbins, G Cheemungtoo, S Tingle, B Mahendran, L Bates, A Amer, G Sen, J Hammond, R Figueiredo, D Talbot, D Manas, S White, N Sheerin, S Ali, C Wilson
Rok vydání: 2023
Předmět:
Zdroj: British Journal of Surgery. 110
ISSN: 1365-2168
0007-1323
DOI: 10.1093/bjs/znad101.049
Popis: Background Kidney transplantation is limited by the number of transplantable organs. Biomarkers that can predict organ viability will reduce the number of organs currently discarded (13% UK, 2021-22, NHSBT). The kidney microcirculation can be flushed, prior to transplantation, with preservation solution as part of the back-table preparation. The effluent fluid is usually discarded, but we propose this as a source of potential biomarkers. Interleukin-16 (IL-16) is a pro-inflammatory cytokine previously shown to predict viability in liver grafts. Here we examine IL-16 in the context of kidney viability. Methods IL-16 concentration was measured, in the flush effluent, using an electrochemiluminescent multiplex assay. The total protein content of each sample was concurrently measured to normalise the concentration. IL-16 concentrations were then compared to donor characteristics and early transplant outcomes. Results Samples were collected from 30 transplanted kidneys (10 DCD, 10 DBD, 10 live donor) and 5 declined kidneys (4 DCD, 1 DBD). IL-16 concentration was significantly higher in DCD kidneys (mean 81.8+/-38 (SD)) compared to live donor kidneys (17.7+/-18 pg/ml) (p=0.0001). CIT did not affect IL-16 concentration (β=-0.014, p=0.4), nor was IL-16 able to predict delayed graft function. However, IL-16 was significantly higher in kidneys declined for transplantation (transplanted: 54.7+/-30 vs non-transplanted: 98+/-43, p=0.03). Conclusion Flush effluent is a rich source of potential biomarkers. IL-16 concentration in flush effluent fluid correlates with organ quality. With real-time assessment, it will be possible to identify kidneys currently discarded; that are either transplantable directly or would benefit from advanced therapy prior to transplantation.
Databáze: OpenAIRE
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