Isolation of Mucoprotein in Bovine Skeletal Muscle

Autor: Elaine Nelson McINTOSH
Rok vydání: 1965
Předmět:
Zdroj: Journal of Food Science. 30:986-990
ISSN: 1750-3841
0022-1147
DOI: 10.1111/j.1365-2621.1965.tb01874.x
Popis: SUMMARY A mild method for the isolation of mucoprotein from bovine skeletal muscle is described. Minced skeletal muscle was extracted twice with neutral 0.6M KCl. The washed stromal residue was extracted twice with acetone, air-dried, and pulverized. The stromal residue powder was extracted twice with 10% CaCl2. The pooled CaCl2 extracts were added to 1.5 volumes ice-cold ethanol and refrigerated. The crude mucoprotein precipitate was obtained by centrifugation, and suspended in 5% sodium acetate. After extraction for 24 hr at 4°C, the mucoprotein suspension was filtered through Celite and purified. The final dried product was pulverized to a light tan powder. Dissolved in phosphate buffer, it gave a viscous milky-white solution. Quantitative hexosamine and nitrogen values obtained at each major step of the isolation procedure are given, and their significance is discussed. Less than 10% of the total stromal residue hexosamine is ex-tractable by CaCl2. The CaCl2-extractable fraction was isolated and purified and designated the “soluble mucoprotein fraction.” Hydrolysates of this fraction were analyzed by paper chromatography. The consistent observation of galactosamine and glucuronic acid indicated that chondroitin sulfate was present. Glucosamine was also noted consistently, along with galactose, but their presence was not easily explained.
Databáze: OpenAIRE
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