The development of iNKT cells is dependent on a full complement of functional CD3 ζ ITAMs (36.39)
| Autor: | Jennifer Eitson, Amy Becker, Michael Norgard, Nicolai van Oers |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 184:36.39-36.39 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Invariant natural killer T (iNKT) cells are involved in the early phase of an immune response and are proposed necessary for reducing the severity of Lyme disease during Borrelia burgdorferi infection. Little is known about the number of functional TCR encoded ITAMs required for iNKT cell selection and expansion. We analyzed iNKT cell development in CD3 ζ transgenic lines with various tyrosine to phenylalanine substitutions (YF) that eliminated some or all phospho-ζ intermediates. iNKT cell numbers were significantly reduced in the thymus, spleen, and livers of the YF lines compared to wild type mice. Interestingly, the extent of cardiac tissue damage and inflammation in response to B. burgdorferi infections was similar in the YF lines compared to wild type controls, and even mice lacking all iNKT cells (Ja18-null animals). The relative expression of two key transcription factors, Egr2 and PLZF, was significantly reduced in the YF lines. This suggests that the magnitude of TCR/ITAM-driven signals controls the expression of two key transcription factors essential for iNKT cell development. Thus, while a full complement of functional CD3 ζ ITAMs is required for iNKT cell development, these cells are not essential for controlling arthritis or carditis following B. burgdorferi infections. |
| Databáze: | OpenAIRE |
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