Preparation of Sm-doped CaZrO3 nanosheets for facile human serum exosome isolation
| Autor: | Guangyao Wu, Hongchao Geng, Qingyun Cai, Ping Chen, Chenyi Li, Jie Song |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Chemistry 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology Isolation (microbiology) Exosome Catalysis Microvesicles 03 medical and health sciences Biochemistry Basic research Potential biomarkers Materials Chemistry TSG101 0210 nano-technology 030304 developmental biology |
| Zdroj: | New Journal of Chemistry. 45:11719-11726 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/d1nj01055g |
| Popis: | Cell-derived exosomes from human serum are potential biomarkers for the early diagnosis of diseases. Rapid and effective isolation of high-purity exosomes from human serum is essential for both basic research and clinical applications. Herein, we proposed a new strategy for the simple, rapid and effective isolation of exosomes from human serum by making the best of the high affinity of Sm-doped CaZrO3 (CaZrO3:Sm) with the phosphate groups on the lipid bilayer of exosomes. Compared with the commercially available TiO2, the proposed CaZrO3:Sm achieves a higher enrichment efficiency for target proteins CD63 and TSG101. In addition, a total of 20 protein spots were identified from the human serum after enrichment by CaZrO3:Sm. Moreover, CaZrO3:Sm still has satisfactory enrichment efficiency after being reused 3–4 times. The results demonstrate the feasibility of the novel strategy as a forceful tool for exosome isolation for health monitoring and disease diagnosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|