Fourier Transform Mid-Infrared (FTIR) Spectroscopy to Identify Tannin Compounds in The Panicle of Sorghum Mutant Lines

Autor: Anuraga Jayanegara, I Komang Gede Wiryawan, Irawan Sugoro, Dewi Apri Astuti, Teguh Wahyono
Rok vydání: 2019
Předmět:
Zdroj: IOP Conference Series: Materials Science and Engineering. 546:042045
ISSN: 1757-899X
1757-8981
DOI: 10.1088/1757-899x/546/4/042045
Popis: G5 and G8 are two sorghum mutant lines projected as sorghum varieties for ruminant’s feed. However, most of the sorghum panicles contains phenolic compounds, especially in the form of condensed tannins. The objective of this research was to investigate the phenolic and tannin compounds from the panicles of two sorghum mutant lines. The second objective was to identify tannin content by Fourier Transform Mid-Infrared (FTIR) Spectroscopy in the sorghum panicles. The two mutant lines were compared with Pahat variety. This research was arranged into a randomized complete block design with two factors. The first factor was the sorghum variety/mutant lines, whereas the second factor was the generative phase of harvesting time (flowering, soft dough and hard dough). The results showed that the panicles of G5 sorghum contained the lowest total phenol compounds (P−1. At 1521-1514 and 1446-1408 cm−1, a band due to the C-C aromatic compounds was observed. Based on those findings, it can be concluded that G5 and G8 mutant lines produced less total phenol and total tannin contents than Pahat variety. Meanwhile, FTIR could be used to describe the profile of tannin compounds qualitatively.
Databáze: OpenAIRE