Development and monitoring of purification process for nerve growth factor fusion antibody

Autor:	R.A Hamilton, Neal F. Gordon, A Schutz, S. D. Putney, P.D Lynch, A.J Hunt, X Cao, Tom Londo, T McCormack, P Dimond, M Percoskie, J.P McGrath
Rok vydání:	1995
Předmět:	Fusion Chromatography biology Chemistry Chinese hamster ovary cell Organic Chemistry General Medicine Biochemistry Analytical Chemistry law.invention Tissue culture Nerve growth factor law Recombinant DNA biology.protein Antibody Fetal bovine serum Monitoring procedure
Zdroj:	Journal of Chromatography A. 708:61-70
ISSN:	0021-9673
DOI:	10.1016/0021-9673(95)00376-x
Popis:	A purification process and an on-line process monitoring procedure for a human nerve growth factor (NGF) fusion antibody (NAK) was developed. The NAK protein was produced with a recombinant technique by an adherent Chinese hamster ovary cell line in a tissue culture system supplemented with 5–10% fetal bovine serum, then purified from tissue culture supernatant. The overall purity goal for the process was 90%. A capture step was developed and optimized on POROS 50A which served as a purification and concentration step for the NAK product. A second purification step to remove contaminating bovine IgGs was developed and optimized first on a POROS HS/M cation-exchange column using 20-μm particles. The process was then scaled to a POROS 50 HS (50-μm particle) column. Once an optimized process was developed the capture step on POROS 50A was scaled from an 8-ml column to a 0.3-1 column on PerSeptive Biosystems AutoPilot system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3811a9c687c175fa615e125825fbae01 https://doi.org/10.1016/0021-9673(95)00376-x Zobrazit plný text záznamu Full Text from ScienceDirect