Fluorescent probe for simultaneous discrimination of Cys/Hcy and GSH in pure aqueous media with a fast response under a single-wavelength excitation
| Autor: | Xiaojie Ren, Long He, Xiangzhi Song, Liu Yang, Huihui Tian, Xingjiang Liu, Yani Geng |
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| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Fluorophore Chemistry Metals and Alloys 02 engineering and technology Glutathione 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Materials Chemistry Biophysics Reactivity (chemistry) Electrical and Electronic Engineering 0210 nano-technology Selectivity Instrumentation Excitation Cysteine |
| Zdroj: | Sensors and Actuators B: Chemical. 273:1170-1178 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2018.04.163 |
| Popis: | Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are of great importance in many physiological and pathological processes. Due to their similar structure and reactivity of these three biothiols, it is a great challenge to simultaneously distinguish one from another. By virtue of two different fluorescence signals, nitrobenzofurazan (NBD) has been proved to be an effective sensing functional group as well as a green fluorophore precursor to construct fluorescent probes for simultaneous discrimination of Cys/Hcy and GSH. However, most of the reported NBD-based fluorescent probes suffered from a poor water-solubility, a slow response, double-wavelength excitation or two interfering fluorescence signals (narrow wavelength separation in λmaxEm and/or big difference in ΦFl). In this study, we developed a fluorescent probe, MCQ-O-NBD, for simultaneous discrimination of Cys/Hcy and GSH using two matched fluorescent signals (well separated red and green fluorescence and comparable ΦFl), which could function in a pure aqueous media with a rapid response under a single-wavelength excitation. This probe exhibited high sensitivity and good selectivity as well as low detection limit, and had been successfully applied to visualize and differentiate Cys/Hcy and GSH in living cells. |
| Databáze: | OpenAIRE |
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