Tragia plukenetii Radcl.-Sm.: Anticancer, anti-migration potential on A549 cell line and molecular docking analysis on Lung cancer receptors – A systematic study

Autor: Sowmya Priya Manoharan, Yadav Sangilimuthu Alagar, Suvathika Gnanaselvan, Soundara Rajan Thangavelu, Samy Venkataa Suresh Muni
Rok vydání: 2023
Předmět:
Zdroj: Research Journal of Biotechnology. 18:79-89
ISSN: 2278-4535
0973-6263
Popis: Lung cancer is a terrible disease with highest mortality. Although, several modern medications saved many lives but side effects remain dreadful. The natural remedy is needed to overcome the side effects of the lung cancer treatment. Tragia plukenetii belongs to Euphorbeaceae family reported for good ethnopharmacological property which remains unexplored in its anticancer prognosis. This study focusses on cytotoxicity analysis of T. plukenetti extract against A549 cell line and L929 by MTT assay. Scratch assay was done to evaluate their anti-migratory effect against A549 cell line. GC-MS techniques were used to analyse the identification of phytocompounds present in the T. plukenetii leaves and screened their anti-lung cancer mechanism targeting Lung cancer receptors (EGFR, RET, ALK, BRAF, JAK3 and P13k) through molecular docking by SeeSAR 9.2. commercial tool. Best docked ligands were subjected to ADMET analysis and Swiss Target Prediction to check the drug likeliness properties of the ligands. MTT analysis of T.plukenetii leaves extracts against A549 lung cancer cell line revealed the IC50 value as 91.53 ± 0.2 μg and less cytotoxic effect in L929 cell lines. Scratch invasion assay showed 88% inhibition in wound closure at 36 Hrs. T. plukenetii contains fifteen volatile compounds with as Z, Z-3,13-Octadecadien-1-ol (25.71), Oleic acid (16.21) 9-Eicosyne (15.72) and 9,12- Octadecadienoic acid (Z,Z)-(12.62) as major metabolites in the GC-MS analysis. Among best docked ligands, oxacyclododecan-2-one obeyed the major ADMET categories. Top 15 receptor targets predicted from Homo sapiens origin showing T. plukenetii have the anti-cancer potential that may pave the way for the drug discovery.
Databáze: OpenAIRE
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