Nucleolar localization of BRCA1 protein in human breast cancer

Autor:	Ira J. Bleiweiss, M Mendlowitz, Leonard Ornstein, E Weinstein, Steven Dikman, Natalie Tulchin
Rok vydání:	1998
Předmět:	Cancer Research biology medicine.drug_class Monoclonal antibody medicine.disease Molecular biology Staining chemistry.chemical_compound Oncology Antigen retrieval chemistry Polyclonal antibodies Monoclonal biology.protein medicine Cancer research Immunohistochemistry Papilloma Antibody
Zdroj:	International Journal of Oncology.
ISSN:	1791-2423 1019-6439
DOI:	10.3892/ijo.13.3.513
Popis:	Both a rabbit polyclonal BRCA1 antibody, K-18, and a mouse monoclonal BRCA1 antibody, AP 16, produced nucleolar epithelial cell staining on frozen tissue sections of human infiltrating mammary carcinomas. There was much less BRCA1 antibody staining in normal tissues; however, 2 intraductal tumors and a papilloma, found in proximity to the carcinomas showed considerable nucleolar immunoreactivity. MCF-7 cells fixed in methanol and immunostained with the same two antibodies also revealed nucleolar staining, however, after 4% paraformaldehyde fixation for three minutes, there were many fewer nuclei stained. Antigen retrieval methods on formalin-fixed, paraffin-embedded specimens produced tumor cell cytoplasmic staining with AP 16 and nuclear staining in both tumor and normal epithelial cells with another BRCA1 monoclonal antibody, SG 11.
Databáze:	OpenAIRE
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