| Popis: |
Non-toxic level of reactive oxygen species (ROS) has been reported to be involved in transducing intracellular signals and thereby regulating fundamental cell behaviors such as proliferation, differentiation and survival. Previous study showed that ROS is highly generated in pre-adipocytes and be involved in modulating pre-adipocyte differentiation. In this study, the suppressive effect of CAPE on 3T3-L1 differentiation to adipocyte through the inhibition of ROS generation was investigated. Cells were stained with Oil-Red-O to detect oil droplets in adipocytes. Also, the expression of adipogenic transcription factors such as CCAAT/enhancer binding protein α (C/EBPα) and peroxisome proliferator activated receptor γ (PPAR γ) was observed by western blot. Lipid accumulation in the mature adipocyte was significantly suppressed by CAPE treatment between 10 and 50 μM in a dose-dependent manner. PPARγ and C/EBPα known to be associated with adipogenic transcription factor in adipocytes were significantly attenuated by CAPE treatment. Intracellular ROS level increased to its highest level within 2 days and was dose-dependently reduced by CAPE treatment. In addition, the expression of NADPH oxidase 4 (NOX4), p22phox was suppressed and the translocation of p47phox to membrane by CAPE treatment. |
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