| Autor: |
Sang Hoon Kim, Y.B. Hahn, Rafiq Ahmad, S. W. Hwang, Ali Al-Hajry, Ahmad Umar |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Electrochimica Acta. 135:396-403 |
| ISSN: |
0013-4686 |
| DOI: |
10.1016/j.electacta.2014.04.173 |
| Popis: |
This paper reports the fabrication of highly-sensitive and selective voltammetric/amperometric cholesterol biosensor based on cholesterol oxidase co-immobilized with α -Fe 2 O 3 micro-pine shaped hierarchical structures. The α -Fe 2 O 3 micro-pine shaped hierarchical structures were synthesized by facile hydrothermal process in large quantity and characterized in detail using various techniques. The detailed studies demonstrated that the as-synthesized α -Fe 2 O 3 hierarchical structures are grown in very high density exhibiting well-crystallinity and demonstrating rhombohedral α-Fe 2 O 3 crystal structures. The fabricated cholesterol biosensors based on α -Fe 2 O 3 hierarchical structures exhibited a very high and reproducible sensitivity of 78.56 μA/mMcm 2 and detection limit (based on S/N ratio) of 0.018 mM. The biosensor exhibited a linear dynamic range from 0.1-8.0 mM and correlation coefficient of R = 0.9951. A lower value of apparent Michaelis-Menten constant ( K m app ), of 0.007 mM, exhibited a high affinity between the cholesterol and ChO x immobilized on α -Fe 2 O 3 micro-pine shaped hierarchical structures. To the best of our knowledge, this is the first report in which α -Fe 2 O 3 micro-pine shaped hierarchical structures are used for the fabrication of highly sensitive and selective cholesterol biosensor. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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