Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization
| Autor: | Kuidong Xu, Zifeng Zhan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Scuticociliatia
food.ingredient 010504 meteorology & atmospheric sciences medicine.diagnostic_test Abalone biology Oligonucleotide Ruditapes Aquatic Science 010502 geochemistry & geophysics Oceanography biology.organism_classification 01 natural sciences Silver stain food Biochemistry Morphological analysis medicine Haliotis 0105 earth and related environmental sciences Fluorescence in situ hybridization |
| Zdroj: | Acta Oceanologica Sinica. 39:90-94 |
| ISSN: | 1869-1099 0253-505X |
| Popis: | The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink