A Qualitative and Quantitative Approach to Determine the Optimum Combination of Feeding Stimulants for Striped Bass Morone saxatilis Using an Agar Gel Carrier
| Autor: | Elias Papatryphon, Joseph H. Soares |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Journal of the World Aquaculture Society. 33:23-31 |
| ISSN: | 1749-7345 0893-8849 |
| DOI: | 10.1111/j.1749-7345.2002.tb00474.x |
| Popis: | A series of experiments was conducted to determine the optimum combination of previously identified feeding stimulants (FS), namely L-alanine (Ala), L-serine (Ser), inosine-5′-monophosphate (IMP), and betaine (Bet), for striped bass Morone saxatilis. Three experiments were conducted to determine the optimum combination of FS using an agar gel matrix as a carrier. In the first experiment a 24 factorial experiment was conducted to test all possible combinations of the four FS at two levels, 0 and 0.1 M. Significant interactions between the FS were found, suggesting the complexity of gustatory stimulation and palatability. In the second experiment a 4 × 6 factorial design was employed to test each FS alone and at concentrations ranging from 0 to 8% in order to determine the minimum level at which maximal stimulation is achieved. The results suggest that there is no significant improvement in feed intake beyond the 1 % level of supplementation for all the FS. In addition, Ala produced a significantly greater response compared to all other FS. In the last experiment, a modified single factor method was used to estimate the optimum levels for each FS in a mixture. The range of the concentrations tested was 0–1% of the agar gel for each FS. Combining all four compounds yielded maximal stimulation. The levels of each compound in the final optimum combination of FS were: Ala, 0.4; Ser, 0.6; Bet, 0.4; and IMP, 0.3% of the agar gel. |
| Databáze: | OpenAIRE |
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