Evaluation of Magnetite Nanoparticle-Based Toxicity on Embryo–Larvae Stages of Zebrafish (Danio rerio)
| Autor: | Anna Jurewicz, Sanjay Mathur, Jasleen Kaur Uppal, Sigrun I. Korsching, Shaista Ilyas, Ivan Ivandic |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
animal structures
biology ved/biology fungi ved/biology.organism_classification_rank.species Developmental toxicity Danio biology.organism_classification Cell biology Congo red chemistry.chemical_compound chemistry In vivo embryonic structures Toxicity General Materials Science Model organism Zebrafish Iron oxide nanoparticles |
| Zdroj: | ACS Applied Nano Materials. 3:1621-1629 |
| ISSN: | 2574-0970 |
| Popis: | Iron oxide nanoparticles (NPs) are extensively used in various biomedical applications due to their suitability as carriers of diagnostic and therapeutic agents. Herein, we report on the evaluation of concentration-dependent toxicity studies of iron oxide (magnetite) NPs in zebrafish (Danio rerio). To follow the update of iron oxide NPs in the animal model, particles were functionalized by conjugating a fluorescent dye (Congo red) that serves as an efficient probe to track the uptake and accumulations of nanoparticles from the early life stages of zebrafish. As in vivo model organism to study the diffusion an in vivo toxicity, zebrafish embryos and larvae were treated with different concentrations of CR@Fe3O4 conjugates (100–800 μg/mL) for 4–96 h postfertilization (hpf). Monitoring of mortality, hatching rate, and whole-embryo cellular death showed that incubation of low concentrations of the NPs did not exhibit adverse developmental toxicity during embryonic and larval stages of zebrafish. Minimal toxic ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|