| Autor: |
Chunyan Li, Pei-Ming Wu, Raj K. Narayan, Cameron Mozayan, R. Chabra |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS). |
| DOI: |
10.1109/transducers.2015.7181244 |
| Popis: |
Oxygen gradients play essential roles in many in vitro cell, tissue and organ experimental models. This paper reports a novel biocompatible biomaterial for on-demand generation of three-dimensional oxygen gradients in vitro. By exposing the oxygen-consuming biomaterial consisting of glucose oxidase and catalase enzymes to the cell culture media or gel, precisely-controlled oxygen gradients (2.5 mmHg per 100 µm distance) that closely mimic in vivo hypoxia can be generated. This biomaterial will serve as the basis for a new generation of experimental models previously impossible or very difficult to implement by simplifying and refining the control of in vitro microenvironments. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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