| Autor: |
Shiao-Shek Tang, Jyh-Hwa Kau, Chih-Heng Huang, Chuan-Wang Li, Cheng-Che Liu, Der-Jiang Chiao, Wen-Zhi Lin, Cheng-Cheung Chen, Jiunn-Jye Wey, Rong-Hwa Shyu, Pei-Yi Tsui |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Journal of Medical Sciences. 39:217 |
| ISSN: |
1011-4564 |
| DOI: |
10.4103/jmedsci.jmedsci_203_18 |
| Popis: |
Background and Aim: Botulinum neurotoxin Type E (BoNT/E), one of the most lethal toxin known, is the common contamination in fishery products or fish consumption that causes foodborne botulism. It is necessary to establish a sensitive and specific method for the detection of BoNT/E because of its extremely low lethal dose. Methods: In this study, a practical enzyme-linked immunosorbent assay for BoNT/E detection was developed. The assay is based on the sandwich format using monoclonal antibodies of two distinct specificities. An affinity-purified anti-BoNT/E light chain Mab (1E1) is utilized to adsorb BoNT/E from solution, and the second anti-BoNT/E heavy chain C terminus Mab (5E1) conjugated with peroxidase is then used to form sandwich complexes, and peroxidase allows color development and measurement of optical density at 450 nm. Results: Standard curves were linear over the range of 5–100 ng/ml BoNT/E. The limit of detection was below 10 ng/ml in phosphate-buffered saline buffer. The developed BoNT/E assay also showed no cross-reaction to Type A neurotoxin (BoNT/A) and Type B neurotoxin (BoNT/B). Conclusion: Herein, a sensitive and accurate ELISA for BoNT/E detection was presented. It has the potential to utilize in vivo BoNT/E analysis and contamination monitoring. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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