Popis: |
AMP gene expression following no-wound controls in (A) D. virilis and (B) D. neotestacea. Treatments involved a sterile wound (Sterile), Serratia bacterial challenge (Septic), or a no-wound control (No Wound). Comparing sterile wound treatments to no-wound controls, in D. virilis, AttB was induced 2.9-fold (t(6.79) = –2.22, p = .063), while in D. neotestacea AttB was induced by 4.2-fold (t(13.25) = –4.99, p 0.1). The D. neotestacea DptC was not upregulated by Serratia challenge, even relative to unwounded control flies. Drosocin is not appreciably induced by sterile wounding in either species, despite drosocin being induced by sterile wounding in D. melanogaster (Lemaitre et al., 1997); this difference in expression may be due to drosocin’s shift in genomic position between these two lineages (Fig. 3b). (PDF 439 kb) |