Protective Effect of ATRA on Bleomycin Induced Lung Fibrosis in Rat
| Autor: | Serairi Beji Raja, Abidi Anouar, Jameleddine Saloua, Msilini Najoua, Ksouri Riadh, Saidi Oussama |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Vitamin
chemistry.chemical_classification Pathology medicine.medical_specialty Lung biology business.industry Glutathione peroxidase respiratory system Pharmacology Malondialdehyde medicine.disease Bleomycin respiratory tract diseases Superoxide dismutase chemistry.chemical_compound medicine.anatomical_structure chemistry Catalase Pulmonary fibrosis biology.protein Medicine business |
| Zdroj: | Medicinal Chemistry. 4 |
| ISSN: | 2161-0444 |
| DOI: | 10.4172/2161-0444.1000202 |
| Popis: | All-trans-retinoic acid (ATRA), an active metabolite of vitamin A, is known to affect cell differentiation, proliferation, and development. Pulmonary fibrosis is one of the most common chronic interstitial lung diseases with high mortality rate after diagnosis and limited successful treatment. The aim of this study is to assess the effects of ATRA against bleomycin-induced pulmonary fibrosis in rats. Animals were intratracheally instillated with bleomycin and/or intraperitoneally administered with ATRA. On day 28, rats were sacrificed and histological changes in the lungs were evaluated. Moreover, malondialdehyde (MDA) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in serum and lung, were achieved. Results displayed that rat body weight decreased while fibrosis score and inflammatory index in lung tissue were significantly increased, after bleomycin instillation. Administration of bleomycin followed by ATRA reduced bleomycin–induced weight loss, decreased the lung index and the inflammatory indices. Histopathological examination confirmed the antifibrotic effect of ATRA which apparently attenuated the degree of pulmonary fibrosis. In addition, data showed that ATRA significantly increased SOD, CAT and GPx levels as compared to bleomycin group (G2) concomitant to the decrease of MDA content in lung homogenates. These findings indicate that ATRA treatment significantly attenuated the increased pulmonary damage induced by bleomycin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|